アブストラクト（54巻2号：神奈川歯学）

神奈川歯学

English

Title　： Porphyromonas salivosa ATCC 49407 fimbriae induced osteoclast differentiation and cytokine production
Subtitle　： ORIGINAL ARTICLE
Authors　： Keitaro INABA1), Yasunori KOYATA1), Haruka SASAKI1), Toshizo TOYAMA1), Hiroko HIRAMINE2), Takayuki OGAWA3), Kiyoko WATANABE1), Nobushiro HAMADA1)
Authors（kana）　：
Organization　： 1)Division of Microbiology, Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental University, 2)Department of Highly Advanced Stomatology, Yokohama Clinical Education Center of Kanagawa Dental University, 3)Ogawa Animal Hospital
Journal　： Kanagawa Shigaku
Volume　： 54
Number　： 2
Page　： 92-101
Year/Month　： 2019 / 12
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] Porphyromonas salivosa ATCC 49407 is a black-pigmented, anaerobic, Gram-negative, and rod-shaped organism. P. salivosa is isolated from the gingival sulcus of various animals including dogs and cats. Fimbriae are filamentous components on the cell surface and are thought to play an important role in the colonization of periodontal tissues. We examined the involvement of the fimbrial protein in osteoclast differentiation and cytokine production in murine macrophages. Furthermore, alveolar bone resorption induced by P. salivosa infection in rats was evaluated. Fimbrial protein was purified from P. salivosa by selective protein precipitation and chromatography on a DEAE CL-6B anion exchange column. Western blotting analysis was performed with PAbs against fimbrial protein from P. salivosa. Expression of fimbriae on the surface of P. salivosa was investigated using transmission electron microscopy. To estimate osteoclast differentiation, bone marrow cells and MC3T3-G2/PA6 cells were cultured with or without the purified fimbrial protein. BALB/c mouse peritoneal macrophages were stimulated with the purified fimbrial protein, and cytokine production was determined by ELISA. Sprague-Dawley rats were infected with P. salivosa. Forty-five days after the last infection, the periodontal bone levels were determined by a morphometric measurement. We determined P. salivosa had fimbrial structure on the cell surface, and purified 60-kDa fimbrial protein. Osteoclast differentiation was significantly enhanced with the treatment of the 60-kDa fimbrial protein. The purified fimblial protein induced IL-1β and TNF-α production. Rats orally infected with P. salivosa exhibited significant bone loss compared with that of sham-infected rats. These results suggest that P. salivosa 60-kDa fimbriae may provoke an inflammatory response in host and be involved in periodontal tissue breakdown.
Practice　： Dentistry
Keywords　： fimbriae, periodontal disease, Porphyromonas salivosa, osteoclast differentiation